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KMID : 0380619720040030200
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Korean Journal of Food Science and Technology
1972 Volume.4 No. 3 p.200 ~ p.205
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Production of Single Cell Protein on Petroleum Hydrocarbon
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권태완/Kwon, Tai Wan
변유량/이용현/Pyun, Yoo Ryang/Lee, Yong Hyun
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Abstract
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Effects of several different petroleum fractions (LGO, HGO, VGO, Diesel oil, SP(E), HGO-wax, L/M-wax), stepwise addition of calculated amounts of HGO at defined intervals, recycling of spent media on cell growth of Candida tropicalis KIST 351 were studied using 2.5ℓ fermenter by batch process. In addition, continuous cultivation of the yeast was also performed in the light of biomass production using 28ℓ fermenter with LGO.
1) Cell concentration, yield on the basis of gas oil and n-paraffin with the petroleum fractions were in the range of 11∼15g/ℓ, 10∼12% and 77∼82%, respectively.
2) By stepwise addition of the gas oil, cell concentration and yield on the oil were increased up to 18.9 g/ℓ and 13%, respectively.
3) Spent medium slowed emulsifying ability of hydrocarbon and stimulating effect on the cell growth. Wiehout additional supplementation of Mg^(++) up to 20% of spent medium could be reused, while by adding of the Mg^(++), 50% of medium could be recycled.
4) Optimum condition of continuous cultivation for biomass production was attained at the dilution rate of D=0.1∼0.125 hr^(-1). Maximum yield coefficient on consumed n-paraffin was 0.94 at D=0.1 hr^(-1), however, 24% of supplied n-paraffin in the media was not utilized at this dilution rate.
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